Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production
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چکیده
منابع مشابه
Protoplast Formation and Regeneration from Streptomyces Clavuligerus Nrrl 3585 and Clavulanic Acid Production
Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/ mL), in an osmotic solution for 90 min at 28oC. The frequency of regenerated pro...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2002
ISSN: 1517-8382
DOI: 10.1590/s1517-83822002000400014